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本文用放射性前体掺入法研究红缘层孔菌多糖对小鼠体内核酸及蛋白质合成的影响。结果表明,该多糖较明显地提高了[~3H-Letu]掺入正常小鼠血清蛋白及肝脏蛋白的速率,对部份肝切除小鼠肝脏合成蛋白质的能力也有显著的促进作用;同时,该多糖使[~3H-UR]掺入肝脏脾脏RNA的量对比照组分别增加了12％及26％,但对[~3H-TdR]掺入肝、脾DNA的量无显著影响。红缘层孔菌多糖分别与S-180肿瘤细胞、柯萨奇病毒(CBV_3)及Ⅰ型单纯疱疹病毒(HSV-Ⅰ)进行体外培养,结果表明该多糖对三种细胞的增殖无显著影响。
In this paper, the use of radioactive precursor incorporation Rhodobacter bunge polysaccharide on nucleic acid and protein synthesis in mice. The results showed that the polysaccharide significantly increased the rate of incorporation of [~ 3H-Letu] into serum proteins and liver proteins in normal mice, and significantly promoted the ability of hepatic protein synthesis in partial hepatectomy mice. Meanwhile, The amount of [~ 3H-UR] incorporation into liver spleen RNA increased by 12% and 26% respectively compared with the control group, but had no significant effect on [~ 3H-TdR] incorporation of liver and spleen DNA. The polysaccharide of Rhizobium was cultured in vitro with S-180 tumor cell, Coxsackie virus (CBV_3) and herpes simplex virus type I (HSV-Ⅰ) respectively. The results showed that the polysaccharide had no significant effect on the proliferation of the three cell lines.