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目的 :揭示益肾健脾、活血利水方药拮抗肾小球硬化作用途径。方法 :通过肾小球系膜细胞 (Gmc)体外培养技术 ,运用血清药理学方法 ,观察益肾健脾、活血利水方药对含促肾因子血清作用下Gmc增殖及其细胞外基质的影响 ,并以雷公藤多甙片为阳性药物对照。结果 :益肾健脾 ,活血利水方药在含促肾因子血清作用下同样可显著抑制Gmc增殖和Ⅳ型胶原的分泌 ,且一定程度上优于雷公藤多甙片。结论 :在含促因子血清作用下 ,本法方药同样通过抑制Gmc增殖和细胞外基质成分Ⅳ型胶原的生成 ,显示出良好的肾小球硬化拮抗效应
Objective: To reveal the mechanism of action of Yishen Jianpi, Huoxue Lishui Recipe on glomerulosclerosis. METHODS: The in vitro culture of glomerular mesangial cells (Gmc) and serum pharmacology were used to observe the effects of Yishen Jianpi and Huoxue Lishui Recipe on the proliferation of Gmc and its extracellular matrix under the action of serum containing RAS. The triptolide was used as a positive drug control. RESULTS: Yishenjianpi and Huoxue Lishui Recipe also significantly inhibited the proliferation of Gmc and the secretion of type IV collagen under the action of serum containing the promoting factor, and it was better than Tripterygium wilfordii to a certain extent. Conclusion: In the presence of serum containing pro-angiogenic factors, this method also showed good glomerulosclerosis antagonism by inhibiting the proliferation of Gmc and the formation of type IV collagen in extracellular matrix components.