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本文对遵义医学院病生教研室建立的我国第一株小鼠可移植性肝细胞性肝癌模型(H615)进行了电镜观察。取接种H615瘤源后30—50天,肿瘤生长良好的615系小鼠4只(30代、32代各2只)。快速活杀后取其新鲜瘤组织,按电镜制样常规以戊二醛、锇酸双固定,酒精上行脱水,Epon812包埋,超薄切片机制片,铀铅双染色,透射电镜观察和摄片。同时取健康615系小鼠4只,活杀后取其正常肝组织进行电镜对比观察。电镜下H615瘤组织与正常肝组织比较,其细胞超微形态表现主要是核/质比高,核仁增多、增大,有的可见核仁解离、核内假包含物和空泡。少数核膜凹凸不平,核周间隙呈不规则扩大。细胞质表现不一,多数有发育良好的细胞器和膜结构。线粒体散在,多显示肿胀变性。粗面内质网脱颗粒、量减少。滑面内质网腔扩大、量似增多。可见数量不等、大小不一的脂滴和空泡。有的糖元颗粒增加。有的胞质局部呈现溶解、坏死。瘤细胞间有扩
In this paper, the first mouse transplantable hepatocellular carcinoma model (H615) established in the Department of Pathology of Zunyi Medical College was observed by electron microscopy. Take 30-50 days after inoculation of H615 tumor cell line, 4 mice with good tumor growth (30 passages and 2 passages each). After rapid killing, the fresh tumor tissue was taken. According to the conventional electron microscopy sample preparation, glutaraldehyde, osmium tetroxide double fixation, alcohol dehydration, Epon812 embedding, ultrathin slicing mechanism, uranium and lead double staining, transmission electron microscope observation and radiography . At the same time, 4 healthy 615 mice were taken, and their normal liver tissues were killed and killed for comparison. H615 tumor tissue under electron microscopy compared with normal liver tissue, the ultramicroscopic morphology of the cells mainly nuclear / qualitative ratio of high, increased nucleoli, increased, and some visible nucleoli dissociation, nuclear false inclusions and vacuoles. A small number of nuclear membrane rugged, peritubular space was irregularly enlarged. Different cytoplasm, most well-developed organelles and membrane structure. Mitochondria scattered, more show swelling degeneration. Rough endoplasmic reticulum degranulation, the amount of reduction. Sliding surface endoplasmic reticulum cavity expansion, the amount seems to increase. Visible in varying amounts, different sizes of lipid droplets and vacuoles. Some glycogen particles increase. Some cytoplasmic local dissolved, necrosis. Expand between tumor cells