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近年来,探讨外周血淋巴细胞微核率作为电离辐射所致放射损伤的早期生物学诊断指标,国内进行了大量的工作。一般认为,微核是由染色体断裂残留的无着丝点断片或碎片所形成。微核测定方法简便、迅速,较染色体畸变的分析快30倍,故易于广泛采用和推广。一些实验还表明,一定范围内微核率与照射剂量呈线性关系,故微核测定有可能作为一次大剂量照射后估算辐射损伤程度的一种早期快速诊断方法之一。但对长期慢性小剂量照射后的诊断意义还不肯定。测定微核所用染色方法不同,结果也不一样。为了进一步探讨不同染色法对微核率结果的影响,并从中找出较好的染色方法,我室在原有工作的基础上,对健康献血员、医用X 线工作者以及
In recent years, the investigation of the peripheral blood lymphocyte micronucleus rate as an early biologic diagnostic indicator of ionizing radiation-induced radiation injury has led to a great deal of work in China. It is generally believed that micronuclei are formed by non-centromeris fragments or fragments that have residual chromosomes. The micronucleus assay method is simple and rapid, and it is 30 times faster than the analysis of chromosomal aberrations, so it is easy to be widely used and popularized. Some experiments also show that the micronucleus rate is linear with the irradiation dose within a certain range, so micronucleus assay may be one of the early rapid diagnostic methods for estimating the degree of radiation damage after a large dose of irradiation. However, the diagnostic significance of long-term chronic low-dose irradiation is not yet certain. Different staining methods are used to determine the micronucleus, and the results are not the same. In order to further explore the influence of different staining methods on the micronuclear rate results and find a better dyeing method, our laboratory is based on the original work of healthy blood donors, medical X-ray workers, and