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采用高效液相色谱法,以非那西汀为内标物在λ_(max)=278nm下,研究了血浆及尿中氯霉素的含量。本法测定结果:保留时间:氯霉素7 min,非那西汀11min;最低检测浓度:血样为52.3%μg/ml,尿样为37μg/ml;平均回收率:血样为100.5%,尿样为99.7%;线性范围:血样及尿样均为5～50μg/ml;日内变异系数:血样在1.10%以下,尿样在1.19%以下;日间变异系数:血样在5.3%以下,尿样在5.9%以下;新诺明等药物对测定无干扰。
The contents of chloramphenicol in plasma and urine were studied by HPLC at a concentration of 278 nm with phenacetin as internal standard. The results of the determination of the method: retention time: 7 min chloramphenicol, phenacetin 11min; minimum detection concentration: 52.3% of blood samples for the blood sample / ml, 37μg / ml; average recovery rate: 100.5% The linear range was 5 ~ 50μg / ml for blood samples and urine samples. The intra-day coefficient of variation was below 1.10% for blood samples and below 1.19% for urine samples. The coefficient of variation between days was below 5.3% for blood samples, 5.9% or less; no new drugs and other drugs on the determination of interference.