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以抗旱力弱的毛果绣线菊与抗旱力强的华北绣线菊为试验材料,采用不同程度干旱处理,在测定其光合作用变化的基础上,采用蛋白质组学技术研究两种绣线菊光合作用相关蛋白表达的变化。结果表明:干旱胁迫两种绣线菊光合能力降低,光补偿点和光饱和点降低,说明利用弱光能力提高,华北绣线菊适应弱光环境的能力强于毛果绣线菊。不同水平干旱处理分别鉴定出涉及能量代谢与运输的相关蛋白20种。干旱胁迫下毛果绣线菊中卡尔文循环途径相关酶、光系统Ⅱ中多数放氧复合蛋白和捕光色素复合蛋白、参与光合磷酸化的酶发生显著上调和下调表达;华北绣线菊中参与卡尔文循环代谢相关酶、PSⅠ中反应中心蛋白和参与光合电子传递的蛋白、PSⅡ中捕光色素复合蛋白、参与光合磷酸化与氧化磷酸化的蛋白发生明显的上调和下调表达。干旱胁迫使抗旱力不同的毛果绣线菊和华北绣线菊光合作用相关蛋白均受到影响,毛果绣线菊中多是能量代谢相关蛋白发生显著变化,华北绣线菊中多是能量运输相关蛋白发生显著变化。
Based on the changes of photosynthesis, we studied the effects of two species of Spiraea polymorpha (Drosophila melanogaster) and drought-resistant Merbama lactiflora (Drosophila melanogaster) on the drought resistance of different degrees of drought stress Changes of photosynthesis related protein expression. The results showed that the photosynthetic capacity and photosynthetic diminution of two species of Spiraea indica under drought stress decreased, which indicated that the ability of Spiraea polystachya to adapt to the weak light environment was stronger than that of Spilar. Different levels of drought treatment identified 20 proteins involved in energy metabolism and transport, respectively. Under drought stress, the Calvin pathway-related enzymes, most of the oxygen-consuming complex proteins in photosystem Ⅱ and the light-harvesting pigment complex proteins of photosystem II, were significantly up-regulated and down-regulated in photosynthetic phosphorylation; Participate in Calvin metabolism related enzymes, PS Ⅰ reaction center protein and involved in photosynthetic electron transfer protein, PS Ⅱ in the light-harvesting pigment complex protein involved in photosynthetic phosphorylation and oxidative phosphorylation of protein was significantly up-regulated and down-regulated expression. Under drought stress, photosynthesis related proteins of Spiraea polycarpinna and Spiraea polystachurea were affected by drought stress. In most of Spirodela polymorpha, energy metabolism related proteins were significantly changed, and most of Spiraea polymorpha were energy transport Significant changes occur in related proteins.