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目的从乙型流感流行株中筛选获得可在鼠肺中稳定复制的毒株。方法利用免疫抑制剂甘露聚糖分别联合5株乙型流感临床分离株和1株标准株感染BALB/c小鼠,筛选出可稳定在鼠肺中连续感染传代的毒株,并纯化后感染小鼠,检测其致病力与致死率,最后对病毒核酸进行测序,分析分子特征。结果从5株临床分离株中筛选到1株乙型流感病毒,可在小鼠中稳定感染并致死,经测序比对发现适应过程中该株病毒血凝素(HA)发生2个突变:N212Y和E361K,神经氨酸酶(NA)发生1个位点突变S339F,其中HA E361K是裂解位点。结论成功从临床乙型流感分离株筛选适应获得一株鼠肺适应株,为进一步开展药物和发病机制研究提供了可能。
OBJECTIVE: To screen and clone strains of influenza B that can stably replicate in mouse lung. Methods BALB / c mice were infected with 5 clinical isolates of influenza B virus and 1 standard strain respectively by immunosuppressive agents mannan and screened out the strains which can be stably passaged in the lungs of mice for successive infection. Mouse, test its virulence and lethality, and finally sequenced the virus nucleic acid and analyzed its molecular characteristics. Results One strain of influenza B virus was screened from five clinical isolates, which could be stably infected and lethal in mice. Two mutations were found in the hemagglutinin (HA) of the virus during the process of adaptation: N212Y And E361K, one site mutation S339F occurred in neuraminidase (NA), of which HA E361K was the cleavage site. Conclusion The successful screening from a clinical isolates of influenza B to obtain a strain of mouse lung adapted to provide further possibilities for the study of drugs and pathogenesis.