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目的初步探索衰老标记蛋白30(SMP-30)在人肺组织中的表达以及在慢性阻塞性肺疾病(慢阻肺)发病中的意义。方法纳入2012年1月至6月在江苏省人民医院因肺部肿瘤行肺叶切除术的患者20例。肺组织标本从江苏省人民医院组织库申请获得,为距离癌组织>5 cm的相对正常组织。根据患者肺功能及吸烟情况,将患者分为健康对照组7例,吸烟对照组7例,以及慢阻肺组6例。采用免疫组织化学和蛋白免疫印迹法检测SMP-30在各组肺组织中的细胞定位及含量。结果 SMP-30在人肺组织中主要分布于肺泡巨噬细胞胞浆,慢阻肺组肺泡巨噬细胞及SMP-30阳性细胞数量均明显增多。蛋白免疫印迹法显示肺组织SMP-30蛋白在吸烟非慢阻肺组的含量明显高于健康对照组(2.16±0.23比1.10±0.14,P<0.01)。相较于吸烟非慢阻肺组,慢阻肺组SMP-30蛋白表达进一步增加(4.62±0.97比2.16±0.23,P<0.05)。结论 SMP-30的表达增加可能与肺泡巨噬细胞寿命延长、数量增多有关,有可能是慢阻肺发生发展的一个病理环节。
Objective To explore the expression of SMP-30 in human lung tissue and its significance in the pathogenesis of chronic obstructive pulmonary disease (COPD). Methods Twenty patients undergoing lobectomy for lung tumor at Jiangsu Provincial People’s Hospital from January 2012 to June 2012 were enrolled. Lung tissue samples were obtained from the tissue bank of Jiangsu Provincial People’s Hospital and were relatively normal tissues> 5 cm away from the cancer tissue. According to the patient’s lung function and smoking status, the patients were divided into healthy control group (7 cases), smoking control group (7 cases), and COPD group (6 cases). Immunohistochemistry and Western blotting were used to detect the cellular localization and content of SMP-30 in each group of lung tissues. Results SMP-30 mainly distributed in the cytoplasm of alveolar macrophages in human lung tissues. The numbers of alveolar macrophages and SMP-30 positive cells in COPD group were significantly increased. Western blotting showed that the content of SMP-30 protein in lung tissue was significantly higher in non-COPD smokers than in healthy controls (2.16 ± 0.23 vs. 1.10 ± 0.14, P <0.01). Compared with non-COPD smokers, SMP-30 protein expression was further increased in COPD group (4.62 ± 0.97 vs 2.16 ± 0.23, P <0.05). Conclusion The increased expression of SMP-30 may be related to the prolonged life span and the number of alveolar macrophages, which may be a pathological link in the development of COPD.