论文部分内容阅读
利用微卫星(SSR)标记技术,从600对SSR引物中筛选86对扩增条带清晰的引物,检测了来自我国不同地区37个肉质不同颜色萝卜品种的遗传多样性。86对引物共扩增到976个条带,每对引物扩增出2～17个条带,平均为10.7个,其中多态性条带892个,多态性条带比例为91.39%;共检测出753个基因型,每对引物检测2～20个基因型,平均8.7个,其中有效基因型443.99,有效基因型比例为58.96%;Shannon多态性指数变幅为0.44～2.77,平均1.76。当相似系数为0.81时,可将供试萝卜分成3类,第I类包括6份白色肉质萝卜,第II类包括3份红色肉质萝卜和6份白色肉质萝卜,第III类包括22份红色肉质萝卜。各红色肉质萝卜品种间的遗传相似系数有97%大于0.80,而各白色肉质萝卜品种间的遗传相似系数有91%大于0.80。红色肉质萝卜遗传多样性略低于白色肉质萝卜,红色肉质萝卜与白色肉质萝卜间平均相似系数为0.83,说明不同肉色的萝卜间亲缘关系较密切,在分类上红色肉质萝卜可能是白色萝卜的一个变种。
Using microsatellite (SSR) markers, 86 pairs of SSR primers were screened for 86 pairs of primers, and the genetic diversity of 37 radish cultivars from different regions in China were tested. 86 pairs of primers amplified a total of 976 bands, each pair of primers amplified 2 to 17 bands, an average of 10.7, of which 892 polymorphic bands, the proportion of polymorphic bands was 91.39%; a total of A total of 753 genotypes were detected, with 2 to 20 genotypes detected in each pair, with an average of 8.7, including 443.99 in effective genotype and 58.96% in genotype. The Shannon polymorphism index ranged from 0.44 to 2.77 with an average of 1.76 . When the similarity coefficient is 0.81, the radishes can be divided into three categories, the first category includes six white radish, the second category includes three red radish and six white radish, the third category includes 22 red succulent radish. 97% of the genetic similarity coefficients of red radish varieties were greater than 0.80, while the genetic similarity coefficients of white radish varieties were 91% greater than 0.80. The genetic diversity of red succulent radish was slightly lower than that of white succulent radish, and the average similarity coefficient between red succulent radish and white succulent radish was 0.83, which indicated that the genetic relationships among radishes with different flesh color were close. In the classification, red succulent radish may be one of white radish variant.