通常测定并殖吸虫囊蚴的死活,可采用脱囊试验,或根据囊蚴的活动与结构作出决定。我们应用染色法鉴定囊蚴的死活,操作简便,着色迅速,准确性高。 材料与方法 染液配制将中性红溶于pH7蒸馏水配成1％染液,贮存于棕色滴瓶内备用。另取蒸镏水调节至pH4～5或8～10,配成1％中性红染液作对比观察。 染色程序 取染液稀释10倍,置青霉素瓶中, The determination of the life and death of paragonimus metacercaria is usually carried out using an exfoliative test or a decision based on the activity and structure of the metacercariae. We use the staining method to identify the life and death of metacercaria, easy to operate, rapid coloring and high accuracy. Materials and methods Preparation of dye solution Neutral reddish dissolved in distilled water at pH7 dubbed 1% dye, stored in brown drop bottle spare. Another steaming water adjusted to pH4 ~ 5 or 8 ~ 10, dubbed 1% neutral red dye for comparison. Dyeing procedures take dye diluted 10 times, set penicillin bottle,