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目的研究中国汉族人群基质金属蛋白酶9(maxtrixmetalloproteinase9,MMP9)基因C1562T多态性与急性冠状动脉综合征(acutecoronarysyndrome,ACS)发病的关联性。方法用聚合酶链反应限制性片段长度多态性分析法分析101例经冠状动脉(冠脉)造影确诊的ACS患者MMP9基因的C1562T多态性,以同期冠脉造影阴性、排除冠心病诊断的105例患者为对照组,比较两组间MMP9基因多态性频率分布的差异,并结合造影情况,探讨MMP9基因多态性与ACS发病及冠状动脉狭窄程度的关系。结果ACS患者CT+TT基因型频率(27.7%)明显高于对照组(13.3%),两组差异有统计学意义(χ2=6.567,P=0.01),T等位基因频率在ACS组和对照组分别为14.9%、7.2%(χ2=5.617,P=0.018);MMP9基因C1562T多态性分布与ACS冠脉狭窄程度差异无统计学意义(χ2=0.601,P=0.896)。结论MMP9基因C1562T多态性可能与中国汉族人群ACS有关,MMP9基因1562T等位基因可能是ACS遗传易感性的基因标记之一;MMP9基因C1562T多态性与ACS冠脉狭窄程度无关。
Objective To investigate the association between C1562T polymorphism of matrix metalloproteinase 9 (MMP9) gene and the incidence of acute coronary syndrome (ACS) in Chinese Han population. Methods Polymerase chain reaction restriction fragment length polymorphism analysis was used to analyze the polymorphism of C1562T in MMP9 gene in 101 ACS patients confirmed by coronary artery coronary angiography. The coronary angiography was negative in the same period and the diagnosis of coronary heart disease 105 patients as control group. The difference of MMP9 gene polymorphism frequency was compared between the two groups, and the relationship between MMP9 gene polymorphism and the onset of ACS and the degree of coronary artery stenosis were analyzed. Results The frequency of CT + TT genotype (27.7%) in ACS patients was significantly higher than that in control subjects (13.3%) (χ2 = 6.567, P = 0.01) (Χ2 = 5.617, P = 0.018). There was no significant difference in the distribution of C1562T polymorphism between MMP9 gene and coronary artery stenosis (χ2 = 0.601, P = 0.896). Conclusion The C1562T polymorphism of MMP9 gene may be associated with ACS in Chinese Han population. The 1562T allele of MMP9 gene may be one of the genetic markers of susceptibility to ACS. The C1562T polymorphism of MMP9 gene is not associated with the degree of coronary artery stenosis.