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选取火龙果当年春季抽出的未老熟茎段作外植体 ,以 MS为基本培养基 ,诱导腋芽生长及继代培养时附加 6 - BA和 NAA,6 - BA浓度为 1.0～ 12 .0 mg/L,NAA浓度为 0 .2～ 0 .8m g/L;诱导生根时附加 IBA,IBA浓度为 0 .5 mg/L、 1.0 mg/L、 1.5 mg/L。试验结果表明 :以 MS基本培养基附加 6 - BA2 .0 mg/L+NAA0 .2 mg/L 有利于诱导腋芽生长 ;附加 6 - BA12 .0 mg/L+NAA0 .1mg/L 有利诱导增殖和继代 ;附加 IBA1.5 mg/L 诱导生根最好 ,生根培养 7d后开始生根 ,培养 2 0 d生根率达 96 .7%。将火龙果生根苗移栽到菜园土和细砂基质 ,成活率均达 10 0 % ,以细砂为基质的移栽苗木生长较好 ,说明火龙果的组培苗移栽需要有良好的透水 ,透气条件
The stems of unripe mature stems extracted from the fruit of dragon fruit were taken as explants. MS medium was used as the explants to induce the growth of axillary buds and the additional 6 - BA and NAA in the subculture. The concentration of 6 - BA was 1.0 ~ 12.0 mg / L, NAA concentration was 0.2-2.8 m g / L, and IBA was induced when rooting was induced. The concentrations of IBA were 0.5 mg / L, 1.0 mg / L and 1.5 mg / L, respectively. The results showed that the addition of 6 - BA2 .0 mg / L + NAA0.2 mg / L to MS medium was beneficial to the induction of axillary bud growth; the addition of 6 - BA12 .0 mg / L + NAA0.1 mg / L could induce the proliferation and The generation of IBA 1.5 mg / L was the best, and the rooting started 7 days after rooting. The rooting rate reached 96.7% after 20 days of culture. The rooting seedlings transplanted to garden soil and fine sand matrix, the survival rate reached 100%, fine sand as a substrate transplanting seedlings grow better, indicating that the dragon fruit tissue culture transplants need to have a good water , Ventilation conditions