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为了解输血相关病毒 (TTV)在我国母婴传播的可能及分子病毒学依据 ,应用半巢式 PCR调查我院 1 998年 1～ 1 2月间住院的 87例产妇及其新生儿的 TTV感染情况 ,用克隆后序列分析测定两对母婴同时阳性者 TTV基因序列。结果发现 ,在 87例产妇中有 1 2例用半套式 PCR可检测出 TTV,检出率为 1 3.8% ,其中 4例产妇与新生儿同时阳性 (4.6 % ) ;配对比较两对母婴 TTV序列片段 (1 91 5～ 2 1 85核苷酸片段 )同源性均为 1 0 0 %。结果说明 ,TTV在我国可通过母婴传播的途径感染。此结果丰富了对 TTV传播途径的认识。TTV对胎儿的危害及新生儿生长发育的影响值得进一步研究。
In order to understand the possibility of transmitting TTV in our country and the basis of molecular virology, we detected the TTV infection of 87 pregnant women and their newborns hospitalized from January to February 1998 in our hospital by semi-nested PCR Case, using cloned sequence analysis of two pairs of mother-infant simultaneous positive TTV gene sequence. The results showed that TTV was detected by semi-nested PCR in 12 of 87 maternal cases, with a positive rate of 3.8%, of which 4 maternal and newborn infants were positive (4.6%). Two pairs of mothers and infants TTV sequence fragments (1 91 5 ~ 2 1 85 nucleotide fragments) homology were 100%. The results show that TTV in China can be transmitted through the mother-to-child transmission. This result enriches the understanding of the route of transmission of TTV. TTV harm to the fetus and neonatal growth and development worth further study.