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目的评价利奈唑胺(噁唑烷酮类抗菌药)、万古霉素(胺基糖苷类抗生素)2种抗菌药物对耐甲氧西林金黄色葡萄球菌(MRSA)的体外抗菌活性。方法用琼脂二倍稀释法,测定抗菌药物的最低抑菌浓度(MIC);用肉汤稀释法,测定抗菌药物的最低杀菌浓度(MBC),绘制杀菌曲线(KCs)。结果利奈唑胺对临床分离的98株MRSA的MIC50为1μg.mL-1,MIC90为2μg.mL-1,MBC50为8μg.mL-1,MBC90为32μg.mL-1,敏感率为100%;万古霉素对临床分离的98株MRSA的MIC50为1μg.mL-1,MIC90为1μg.mL-1,MBC50为8μg.mL-1,MBC90为32μg.mL-1,敏感率为100%。随着抗菌药物浓度的升高,其杀菌时间缩短不甚明显,呈现非浓度依赖性的特点。结论利奈唑胺对MRSA的体外抗菌活性与万古霉素相当,均显示非浓度依赖性的杀菌曲线。
Objective To evaluate in vitro antibacterial activity of two antibacterials, linezolid (oxazolidinone antibacterials) and vancomycin (antibiotics aminoglycosides), against methicillin-resistant Staphylococcus aureus (MRSA). Methods The minimum inhibitory concentration (MIC) of antibacterials was determined by agar double dilution method. The minimum bactericidal concentration (MBC) of antibacterials was determined by broth dilution method, and the killing curves (KCs) were drawn. The MIC50 of linezolid was 1μg.mL-1, the MIC90 was 8μg.mL-1, MBC90 was 32μg.mL-1, the sensitivity was 100% The MIC50 of vancomycin against clinical isolates was 1μg.mL-1, MIC90 was 1μg.mL-1, MBC50 was 8μg.mL-1, MBC90 was 32μg.mL-1, and the sensitivity was 100%. With the increase of antimicrobial concentration, the sterilization time is not obvious, showing a non-concentration-dependent characteristics. Conclusion The line antibacterial activity of linezolid to MRSA was comparable to that of vancomycin, showing non-concentration-dependent bactericidal activity.