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目的:制备厚朴超临界CO_2萃取物(SFE-CO_2萃取物)的包合物,并对包合物进行形态学观察及质量控制。方法:以羟丙基-β-环糊精为包合材料,与厚朴SE-CO_2萃取物的质量比为9:1,在一定喷雾条件下进行喷雾干燥,制成包合物;将制得的包合物经过处理后,对其形态进行电镜扫描;建立高效液相色谱法(HPLC)含量测定方法,测定厚朴SFE-CO_2萃取物以及其包合物中厚朴酚、和厚朴酚的含量。结果:利用喷雾干燥法能干燥制备厚朴SFE-CO_2萃取物的包合物;电镜扫描可见SFE-CO_2萃取物为类球形,其周围有羟丙基-β-环糊精笼型结构;采用HPLC法进行含量测定,厚朴SFE-CO_2萃取物包合物中和厚朴酚的百分含量为2.003%,厚朴酚的百分含量为1.573%,厚朴超临界CO_2萃取物中和厚朴酚的百分含量为30.50%,厚朴酚的百分含量为32.84%。结论:喷雾干燥法可用于厚朴SFE-CO_2萃取物的包合物制备;用电镜法和HPLC法可以对包合物的质量进行控制。
OBJECTIVE: To prepare the inclusion complex of supercritical CO_2 extract (SFE-CO_2 extract) from Magnolia officinalis, and observe the morphology and quality control of the inclusion complex. Methods: The hydroxypropyl-β-cyclodextrin was used as the inclusion material, and the mass ratio of the SE-CO 2 extract of Magnolia officinalis was 9:1. Spray drying was carried out under certain spray conditions to make the inclusion compound. The resulting clathrate was processed and its morphology was examined by electron microscopy; a HPLC method was established to determine the extract of holopleu SFE-CO 2 and its clathrates, honokiol, and honokiol Phenol content. RESULTS: The spray-drying method was used to prepare the clathrate of the extract of Magnolia officinalis SFE-CO2. The scanning electron microscopy showed that the SFE-CO2 extract was spherical and surrounded by hydroxypropyl-beta-cyclodextrin cage structure; The content of honokiol was 2.003%, the content of honokiol was 1.573%, the supercritical CO 2 extract of Magnolia officinalis and the thickness was determined by HPLC. The percentage of honokiol was 30.50%, and the percentage of honokiol was 32.84%. Conclusion: The spray drying method can be used to prepare the inclusion complexes of SFE-CO 2 extract from Magnolia officinalis. The quality of inclusion complexes can be controlled by electron microscopy and HPLC.