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目的:建立测定绞股蓝中槲皮素、山柰素和异鼠李素含量的HPLC方法。方法:色谱柱为Hypersil ODS_2(250 mm×4.6 mm,5μm);流动相为甲醇-0.4%磷酸水溶液(50:50,V/V);流速0.78 ml·min~(-1);检测波长为360 nm;柱温为25℃。结果:槲皮素、山柰素和异鼠李素均可达基线分离,其线性范围分别为20.02～100.10μg·ml~(-1)(r=0.999 9),12.16～60.80μg·ml~(-1)(r=0.999 9),1.45～7.26μg·ml~(-1)(r=0.999 8)。平均加样回收率分别为97.32%(RSD=1.09%),97.75%(RSD=1.06%),97.29%(RSD=1.29%)。结论:该方法简单、快速、准确,可用于绞股蓝药材的质量控制。
Objective: To establish an HPLC method for the determination of quercetin, kaempferol and isorhamnetin in Gynostemma. METHODS: The chromatographic column was Hypersil ODS_2 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-0.4% phosphoric acid solution (50:50, V / V) at a flow rate of 0.78 ml · min -1. The detection wavelength was 360 nm; column temperature is 25 ℃. RESULTS: Quercetin, kaempferol and isorhamnetin were baseline-separated and the linear ranges were 20.02 ~ 100.10μg · ml -1 (r = 0.999 9), 12.16 ~ 60.80μg · ml ~ (-1) 1) (r = 0.999 9), 1.45 ~ 7.26 μg · ml -1 (r = 0.999 8). The average recoveries were 97.32% (RSD = 1.09%), 97.75% (RSD = 1.06%) and 97.29% (RSD = 1.29%), respectively. Conclusion: The method is simple, rapid and accurate and can be used for quality control of Gynostemma.