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目的采用免疫组化方法,观察细胞因子信号转导抑制因子-1(SOCS-1)和Bax在冠心病猝死(SCD)者心肌中的表达情况,探讨其对SCD诊断的意义。方法 25例诊断为SCD者心脏样本为实验组,25例非心血管疾病猝死者心脏样本为对照组。应用免疫组织化学方法检测SOCS-1和Bax在心肌中的表达,应用SPSS 17.0软件进行统计处理,组间比较采用秩和检验。结果 SCD猝死者心肌SOCS-1与Bax的表达明显高于对照组,Uc值SOCS-1为5.830 6,Bax为5.573,两种指标组间比较,差异均有显著性意义(P<0.01);SCD组中SOCS-1表达阳性以上且Bax表达弱阳性以上有22例,而正常对照组仅有1例。结论 SOCS-1与Bax均可能参与了细胞凋亡的过程,检测两种指标在心肌中阳性表达,可以为SCD的诊断提供客观依据,且联合检测可提高SCD诊断的特异性。
Objective To investigate the expression of SOCS-1 and Bax in myocardium of sudden cardiac death (SCD) by immunohistochemistry and to explore the significance of SOCS-1 and Bax in the diagnosis of SCD. Methods Twenty-five patients diagnosed as SCD were selected as the experimental group and 25 as the control group. The expression of SOCS-1 and Bax in myocardium was detected by immunohistochemical method. Statistical analysis was performed using SPSS 17.0 software. The rank sum test was used to compare the two groups. Results The expressions of SOCS-1 and Bax in the myocardium of sudden death of SCD were significantly higher than those of the control group. The SOCS-1 of Uc was 5.830 6 and Bax was 5.573. There was significant difference between the two groups (P <0.01). In the SCD group, there were 22 cases with positive SOCS-1 expression and weak positive Bax expression compared to 1 case in the normal control group. Conclusion Both SOCS-1 and Bax may be involved in the process of apoptosis. Detecting the positive expression of these two indexes in myocardium may provide an objective basis for the diagnosis of SCD. Combined detection can improve the specificity of SCD diagnosis.