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目的 :通过检测垂体腺瘤中的凋亡细胞 ,研究新的凋亡抑制基因survivin在垂体腺瘤组织中的表达及其与bcl- 2和 p5 3基因表达的相关性 ,探讨垂体腺瘤的发生与细胞凋亡的关系 ,以及survivin、bcl- 2、p5 3基因在垂体腺瘤AP发生中的调控作用。方法 :应用末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记技术 (TUNEL)和免疫组织化学链霉素抗生物素蛋白 -过氧化酶连接法 (SP法 )检测 8例正常垂体组织和 4 8例不同分化程度的垂体腺瘤组织的细胞调亡和survivin、bcl- 2、p5 3基因的表达。统计学分析采用SPSS10 .0软件系统进行处理 ,检验水准取α=0 .0 5。结果 :本组 4 8例垂体腺瘤标本中 ,4 3例有不同程度的凋亡发生 ,凋亡细胞发生率为 89.6 % (4 3/ 4 8)。在非侵袭性垂体腺瘤组织中细胞凋亡指数 (apoptosisindex ,AI)平均为 12 .6 % ,在侵袭性垂体腺瘤组织中AI为2 .7% ,两者相比差异具有统计学意义 (P <0 .0 5 )。survivin基因在正常垂体组织中不表达 ;4 8例垂体腺瘤组织中 ,32例表达阳性 ,占 6 6 .7% ;其中侵袭性垂体腺瘤survivin基因的阳性表达率为 81.5 % (2 2 / 2 7) ,非侵袭性垂体腺瘤为 4 7.6 % (10 / 2 1) ,两者相比 ,差异有统计学意义 (P <0 .0 5 )。分泌型 (功能性 )垂体腺瘤中survivin基因表达阳性率为
OBJECTIVE: To investigate the expression of survivin in pituitary adenoma tissue and its relationship with the expression of bcl-2 and p53 genes by detecting the apoptotic cells in pituitary adenomas and to explore the occurrence of pituitary adenoma And apoptosis, as well as the regulation of survivin, bcl-2 and p5 3 genes in the pathogenesis of AP in pituitary adenoma. METHODS: TUNEL and dUTP-mediated dUTP nick end labeling (TUNEL) and immunohistochemical streptavidin-peroxidase (SP method) were used to detect the expression of 8 normal pituitary tissues and 48 Apoptosis and expression of survivin, bcl-2, p5 3 genes in pituitary adenomas with different degrees of differentiation. Statistical analysis using SPSS10 .0 software system for processing, test level take α = 0. Results: Among the 48 specimens of pituitary adenoma, 43 cases had different degree of apoptosis and the apoptotic rate was 89.6% (4 3/48). The average apoptotic index (AI) in non-invasive pituitary adenomas was 12.6% and in AI of invasive pituitary adenomas was 2.7%, with statistical significance (P < P <0. 05). The survivin gene was not expressed in normal pituitary tissue. Of the 48 pituitary adenomas, 32 were positive, accounting for 66.7%. The positive expression rate of survivin in invasive pituitary adenoma was 81.5% (2 2 / 2 7), non-invasive pituitary adenoma was 4 7.6% (10/21), the difference was statistically significant (P <0.05). The positive rate of survivin gene expression in secretory (functional) pituitary adenomas was