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作者通过大鼠乌头碱中毒心肌线粒体 CCO 的超微结构定位,观察到乌头碱引起 CCO 的定位紊乱。由于损害程度不同,出现三种情况:(1)CCO 失去在线粒体嵴膜和内膜上的定位,酶反应产物呈自由扩散状态,酶反应强度对照组有所增强或相似;(2)CCO 失去在线粒体嵴膜和内膜上的定位,酶反应强度较对照组明显减弱;(3)在同一线粒体中部分保持 CCO 的嵴膜、内膜定位,部分失去 CCO 在嵴膜和内膜上的定位。实验结果表明,乌头碱干扰了心肌细胞呼吸链的电子传递,抑制氧化磷酸化的正常进行,导致心肌能量代谢障碍。
The author through the aconitine poisoning myocardial mitochondrial CCO ultrastructure localization, observed aconitine cause CCO localization disorder. Due to the different degree of damage, there are three cases: (1) CCO loses its localization on the mitochondrial crest membrane and intima, and the enzyme reaction product is in a free-diffusion state and the enzyme reaction intensity is enhanced or similar in the control group; (2) The mitochondrial cristae membrane and intima localization, enzyme reaction intensity was significantly weaker than the control group; (3) in the same part of mitochondria to maintain CCO crest, endometrial localization, partial loss of CCO in the crest and intima localization . The experimental results show that aconitine interferes with the electron transport of the respiratory chain of myocardial cells and inhibits the normal progress of oxidative phosphorylation, leading to myocardial energy metabolism disorders.