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目的观察肉桂醛(CA)对db/db小鼠胰腺细胞的抗氧化活性的影响,并探讨其降糖机制。方法将21只db/db小鼠随机分为对照组(Con)、CA低剂量组、CA高剂量组,分别予安慰剂,CA低剂量和CA高剂量。灌胃3周后,检测各组血糖、血清和胰腺组织抗氧化酶活性及胰腺细胞线粒体活性等。结果与Con组比较,CA低剂量和CA高剂量组血糖降低(P<0.05);CA高剂量组胰腺抗氧化酶的活性均增强,胰腺细胞线粒体活性氧簇(ROS)量降低(P<0.05);CA低剂量组ROS量与Con组比较,差异无统计学意义(P>0.05)。此外,给药组线粒体膜电位均高于Con组,CA高剂量组琥珀酸脱氢酶(SDH)活性增加(P<0.05)。结论 CA能降低db/db小鼠血糖,其作用机制是通过提高胰腺组织抗氧化酶活性,减少体ROS等自由基的产生,从而保护线粒体功能,保护胰腺细胞。
Objective To observe the effect of cinnamaldehyde (CA) on the anti-oxidative activity of db / db mouse pancreatic cells and to explore its hypoglycemic mechanism. Methods Twenty-one db / db mice were randomly divided into control group, CA low-dose group and CA high-dose group, and received placebo, CA low dose and CA high dose respectively. After 3 weeks of gavage, blood glucose, serum and pancreatic tissue antioxidant enzyme activities and pancreatic mitochondrial activity were measured. Results Compared with Con group, the levels of blood glucose in CA low dose group and CA high dose group were significantly decreased (P <0.05). The activity of antioxidant enzymes in pancreas of CA high dose group was increased and the amount of reactive oxygen species (ROS) in pancreas decreased ). There was no significant difference in the amount of ROS in CA low dose group compared with Con group (P> 0.05). In addition, the mitochondrial membrane potential of the treated group was higher than that of the Con group, and the activity of succinate dehydrogenase (SDH) in the high-dose CA group was increased (P <0.05). Conclusion CA can reduce the blood glucose in db / db mice. The mechanism is that CA can protect mitochondrial function and protect pancreatic cells by increasing the activity of antioxidant enzymes in pancreatic tissues and reducing the production of free radicals such as ROS.