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本实验动态观察了大鼠脾切除及粗制大肠杆菌内毒素攻击后血浆内毒素、肿瘤坏死因子（ＴＮＦ）和ＮＯ－２／ＮＯ－３水平的变化。结果发现：脾切除组较假手术组血浆上述因子均明显升高（Ｐ＜０．０５或Ｐ＜０．０１），且和单纯腹腔注射大肠杆菌内毒素后所引起的改变非常类似。注射内毒素后，切脾前后血浆上述因子无明显差异，但均处在较高水平上，秩相关分析表明：血浆ＮＯ－２／ＮＯ－３与内毒素及ＴＮＦ水平有一定相关性（ｒｓ分别为０４２２及０７２１，Ｐ均＜００１），其中与ＴＮＦ高度相关。作者认为：脾切除后机体免疫力下降，可能导致肠道细菌或毒素移位，诱导产生ＴＮＦ等细胞因子，并协同激活体内Ｌａｒｇ：ＮＯ通路。这也可能是脾切除后凶险性感染（ＯＰＳＩ）发生机制之一。
This experiment dynamically observed the changes of plasma endotoxin, tumor necrosis factor (TNF) and NO-2 / NO-3 levels after splenectomy and crude E.coli endotoxin challenge in rats. The results showed that the above factors in splenectomy group were significantly higher than those in sham operation group (P <0.05 or P <0.01), and were similar to the changes caused by simple intraperitoneal injection of E. coli endotoxin. After injection of endotoxin, there was no significant difference in the plasma levels of these factors before and after splenectomy, but both were at a high level. The rank correlation analysis showed that plasma NO-2 / NO-3 had some correlation with endotoxin and TNF levels 0 422 and 0 721, P all <0 01), which is highly correlated with TNF. The author believes that: spleen resection immunity decreased, may lead to intestinal bacteria or toxins shift, induce TNF and other cytokines, and synergistically activate the body L arg: NO pathway. This may also be one of the mechanisms behind the post-splenectomy dangerous infection (OPSI).