论文部分内容阅读
植物名称:三色绿萝(Epipremnum aureum cv.Tricolor)。材料类别:茎尖、幼嫩的茎段。剪取三色绿萝的顶芽约1cm长,用70％酒精和0.1％升汞表面消毒后,在无菌操作下剥离茎尖约1～2 mm,同时横切茎段1～2mm作为外植体。培养条件:诱导不定芽的培养基:MS+BA 1～2 mg/L(单位下同)+NAA 0.2;诱导愈伤组织培养基:MS+NAA2+BA 0.5;诱导芽分化培养基:MS+BA2+GA_30.1+IAA 0.05;生根培养基:1/2 MS+IBA 1。培养基均为蔗糖3％(生根培养基
Plant name: Epipremnum aureum cv.Tricolor. Material category: shoot tip, tender stem segments. Cut the top buds of Trifolium pratense about 1cm long, and then sterilize the stem tips about 1 ~ 2mm under aseptical operation with 70% alcohol and 0.1% mercuric chloride, and transversely cut stems 1 ~ 2mm as explants body. Incubation medium: MS + BA 1 ~ 2 mg / L + NAA 0.2; Induction callus medium: MS + NAA2 + BA 0.5; Induction sprout differentiation medium: MS + BA2 + GA_30.1 + IAA 0.05; rooting medium: 1/2 MS + IBA 1. The medium is sucrose 3% (rooting medium