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目的探讨抵当汤及分别加入柴胡、桂枝、黄芪3个加味方对S180荷瘤小鼠外周血IL-8、TNF-α的影响。方法小鼠造模后随机分为模型组、抵当汤组(4 g/kg)、抵当汤加黄芪组(5.67 g/kg)、抵当汤加桂枝组(5.5 g/kg)、抵当汤加柴胡组(5 g/kg),每组15只,另取正常小鼠10只作正常对照组。各用药组灌胃给予相应药物,正常对照组和模型组给等体积0.9%NaCl溶液,每日1次,连续10 d。末次给药后1 h于小鼠眶后静脉取血,离心取血清,采用ELISA方法检测IL-8、TNF-α。结果与正常对照组比较,模型组小鼠血清IL-8水平显著升高(P<0.05);与模型组比较,抵当汤可明显降低S180荷瘤小鼠血清IL-8水平(P<0.05),抵当汤加柴胡组、抵当汤加黄芪组、抵当汤加桂枝组均可明显降低S180荷瘤小鼠血清IL-8水平(P<0.05,P<0.01),其中以抵当汤加柴胡组血清IL-8水平降低最显著(P<0.01)。与正常对照组比较,模型组小鼠血清TNF-α水平明显降低(P<0.05);与模型组比较,抵当汤可明显升高S180荷瘤小鼠血清TNF-α水平(P<0.05),抵当汤加柴胡组、加黄芪组、加桂枝组均可明显升高S180荷瘤小鼠血清TNF-α水平(P<0.05,P<0.01),其中以抵当汤加柴胡组血清TNF-α水平升高最显著(P<0.01)。结论抵当汤及抵当汤加味方能升高S180荷瘤小鼠血清TNF-α水平和降低血清IL-8的表达,其中又以抵当汤加柴胡组效果最为显著。
Objective To investigate the effects of dengtang decoction and the added prescription of Bupleurum chinense, Guizhi and Astragalus on IL-8 and TNF-α in peripheral blood of S180 tumor-bearing mice. Methods The mice were randomly divided into three groups: model group, Dangdang Decoction group (4 g / kg), Dangtang Astragalus group (5.67 g / kg), Dangguiganji group (5.5g / kg) (5 g / kg), each group of 15, another 10 normal mice as a normal control group. Each drug group was given gavage appropriate drugs, normal control group and model group to an equal volume of 0.9% NaCl solution, once a day for 10 days. Blood samples were taken from the retro-orbital vein of mice 1 h after the last administration, and serum was collected by centrifugation. IL-8 and TNF-α were detected by ELISA. Results Compared with the normal control group, serum IL-8 level in the model group was significantly increased (P <0.05). Compared with the model group, (P <0.05, P <0.01). The serum level of IL-8 in serum of S180-bearing mice was significantly lower than that of the control group -8 levels decreased the most significant (P <0.01). Compared with the normal control group, the level of serum TNF-α in the model group was significantly decreased (P <0.05). Compared with the model group, the serum level of TNF-α in the S180 tumor-bearing mice was significantly increased (P <0.05) Serum levels of TNF-α in S180 tumor-bearing mice (P <0.05, P <0.01) were significantly higher than those in Tongji Bupleurum group, Astragalus membranaceus group and Guizhi group The level of the most significant increase (P <0.01). Conclusion Dandang Decoction and Jiaotang Decoction can increase the level of serum TNF-α and decrease the level of IL-8 in S180 tumor-bearing mice.