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采用微机械加工技术制作了具有三维结构的微腔型传感器芯片(腔深300μm,铂工 作电极1.2×1.2mm2)。作者对微腔型电极进行了电化学特性的表征。并通过明胶包埋法将 半乳糖氧化酶固定在电极中,构成微腔型半乳糖传感器。该酶电极对半乳糖测定的上限为 4.5mmol/L,相关系数为0.9980。较适宜的pH范围为7.0~7,5,响应时间<50 s。该酶电极 在14天内连续使用,响应电流无明显变化。放置一个月后测试仍保持较好的电流响应。
Micro-cavity sensor chip with three-dimensional structure (cavity depth 300μm, platinum working electrode 1.2 × 1.2mm2) was fabricated by micro-machining technology. The authors characterize the electrochemical properties of microcavity-type electrodes. The gelatin-embedded method was used to immobilize galactose oxidase in the electrode to form microcavity-type galactose sensor. The upper limit of this enzyme electrode for galactose determination was 4.5 mmol / L with a correlation coefficient of 0.9980. The most suitable pH range is 7.0 ~ 7,5, response time <50 s. The enzyme electrode was used continuously for 14 days without any significant change in response current. After one month of testing, the test still maintained a good current response.