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目的:探讨雌激素对阿尔茨海默病(Alzheimer’s disease,AD)模型大鼠学习记忆能力的影响。方法:选取雌性SD大鼠24只,随机分为假手术组、卵巢切除组(ovariectomy,OVX)、OVX+苯甲酸雌二醇组(estradiolbenzoate,EB),每组8只。于海马注射Aβ1-42建立AD大鼠模型,通过Morris水迷宫观察大鼠的学习记忆能力,同时用ELISA检测脑组织超氧化物歧化酶(super oxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、乙酰胆碱酯酶(acetylcholine esterase,ACh E)的活性,用免疫组化分析神经元型一氧化氮合酶(n NOS)并测定其OD值。结果:与OVX组比较,OVX+EB组逃避潜伏期明显缩短(P<0.05),原平台象限活动时间明显增加(P<0.05),穿越原平台次数明显增多(P<0.05)。雌激素作用还提高大鼠脑组织SOD、ACh E和n NOS活性,降低MDA活性(P<0.05)。结论:研究表明雌激素可改善AD模型大鼠的学习记忆能力,其机制可能通过提高脑组织SOD、ACh E和n NOS活性,降低MDA活性有关。
Objective: To investigate the effect of estrogen on learning and memory in Alzheimer’s disease (AD) model rats. Methods: Twenty-four female Sprague-Dawley rats were randomly divided into sham operation group, ovariectomy (OVX) and OVX + estradiol benzoate (EB) groups. AD model was established by injection of Aβ1-42 in the hippocampus. The learning and memory abilities of rats were observed by Morris water maze. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) ), Acetylcholine esterase (ACh E) were measured. The expression of nNOS was detected by immunohistochemistry and the OD value was measured. Results: Compared with OVX group, the escape latency of OVX + EB group was significantly shorter than that of OVX + EB group (P <0.05). The activity time of quadrant in OVX + EB group was significantly increased (P <0.05) and significantly increased (P <0.05). Estrogen also increased the activity of SOD, ACh E and n NOS in rat brain and decreased the activity of MDA (P <0.05). Conclusion: The results show that estrogen can improve the learning and memory abilities of AD model rats. The mechanism may be related to the activity of SOD, ACh E and n NOS and the decrease of MDA activity.