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目的探讨复方紫红参多糖(CPGP)对小鼠肝癌H_(22)实体瘤的抑制作用及机制。方法 ICR雄性小鼠60只,除空白组外,其余小鼠建立H_(22)荷瘤小鼠模型,随机分为模型组、环磷酰胺(CTX)组(25μg/g)和复方紫红参多糖(CPGP)低、中、高剂量组(70、140、280μg/g),每组10只。各组给予相应干预,共10天。观察小鼠一般状况,计算抑瘤率、脏器指数;ELISA法测定血清TNF-α、IL-2、IFN-γ等指标;采用流式细胞术对脾脏组织T淋巴细胞亚群进行测定。结果环磷酰胺(CTX)组和CPGP低、中、高剂量组对肝癌H_(22)的抑瘤率分别为65.01%、40.19%、51.32%、57.84%。与模型组比较,CPGP各组显著提高小鼠体质量、免疫器官指数(P<0.05,P<0.01),CPGP高剂量组能够升高血清中TNF-α、IL-2、IFN-γ(P<0.05),CPGP各组脾T淋巴细胞亚群CD4~+/CD8~+的比值升高(P<0.01);而CTX组小鼠体质量、免疫器官指数,TNF-α、IL-2、IFN-γ水平及脾T淋巴细胞亚群CD4~+/CD8~+的比值均显著降低(P<0.05,P<0.01)。与CTX组比较,CPGP小鼠体质量、免疫器官指数,TNF-α、IL-2、IFN-γ水平及脾T淋巴细胞亚群CD4~+/CD8~+的比值均显著升高(P<0.05,P<0.01)。结论 CPGP对小鼠肝癌H_(22)具有明显的抑制作用,其机制可能与提高机体免疫功能有关。
Objective To investigate the inhibitory effect of compound fugu ginseng polysaccharide (CPGP) on H_ (22) solid tumor in mice and its mechanism. Methods Sixty ICR male mice were randomly divided into model group, cyclophosphamide (CTX) group (25μg / g) and compound fugu polysaccharide (CPGP) low, middle and high dose group (70,140,280μg / g), 10 rats in each group. All groups were given corresponding interventions for 10 days. The general condition of the mice was observed and the tumor inhibition rate and organ index were calculated. The indexes of serum TNF-α, IL-2 and IFN-γ were measured by ELISA. The T lymphocyte subsets of spleen were determined by flow cytometry. Results The inhibitory rates of CTX and CPGP low, medium and high dose groups were 65.01%, 40.19%, 51.32% and 57.84%, respectively. Compared with the model group, CPGP groups significantly increased the body weight and immune organ index of mice (P <0.05, P <0.01). CPGP high dose group could increase the levels of TNF-α, IL-2 and IFN- <0.05). The ratio of CD4 ~ + / CD8 ~ + in splenic T lymphocyte subsets increased in CPGP group (P <0.01), while the body weight, immune organ index, TNF- IFN-γ and the ratio of CD4 ~ + / CD8 ~ + in splenic T lymphocyte subsets were significantly decreased (P <0.05, P <0.01). Compared with CTX group, the body weight, immune organ index, TNF-α, IL-2, IFN-γ and the ratio of CD4 ~ + / CD8 ~ + in splenic T lymphocyte subsets were significantly increased in CPGP mice (P < 0.05, P <0.01). Conclusion CPGP has a significant inhibitory effect on the H_ (22) hepatocarcinoma in mice, which may be related to the enhancement of immune function.