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目的检测人巨细胞病毒(HCMV)感染在颅咽管瘤组织中的表达,探讨HCMV感染与颅咽管瘤发生的病因学关系。方法采用免疫组化方法检测HCMV立刻早期蛋白1-72(IE1-72)和磷酸化糖蛋白65(pp65)抗原在89例颅咽管瘤组织及10例正常脑组织中的表达情况。结果在颅咽管瘤组织中HCMV IE1-72和pp65抗原表达阳性率分别为77.5%和84.3%,而在正常脑组织中两抗原均为阴性,IE1-72和pp65抗原表达阳性率在颅咽管瘤组织和正常脑组织间均有显著性差异(P=0.000,P=0.000)。IE1-72抗原在牙釉质型和鳞状乳头型颅咽管瘤组织中阳性率分别为83.6%和67.6%,pp65抗原分别为89.1%和76.5%;两抗原在牙釉质型颅咽管瘤组织中的阳性率均略高于鳞状乳头型,但均无统计学差异(P=0.135,P=0.197)。结论 HCMV IE1-72和pp65抗原在颅咽管瘤组织中均有高表达,而在正常脑组织中无表达,HCMV感染及其抗原表达可能与颅咽管瘤的发生有一定关系,其致病机制尚需进一步研究。
Objective To detect the expression of human cytomegalovirus (HCMV) infection in craniopharyngioma tissues and to explore the etiological relationship between HCMV infection and the occurrence of craniopharyngioma. Methods Immunohistochemistry was used to detect the expression of immediate early protein 1-72 (IE1-72) and phosphorylated glycoprotein 65 (pp65) in 89 craniopharyngioma tissues and 10 normal brain tissues. Results The positive rates of HCMV IE1-72 and pp65 antigen expression in craniopharyngioma tissues were 77.5% and 84.3%, respectively, but negative in normal brain tissue. The positive rates of IE1-72 and pp65 antigen expression in craniopharyngioma Tumor tissue and normal brain tissue were significantly different (P = 0.000, P = 0.000). The positive rates of IE1-72 antigen in enamel and squamous papillary craniopharyngioma tissues were 83.6% and 67.6%, respectively. The pp65 antigens were 89.1% and 76.5% respectively. The positive rate of IE1-72 antigen in enamel-type craniopharyngioma tissues In the positive rate were slightly higher than the squamous papillary type, but no statistically significant difference (P = 0.135, P = 0.197). Conclusion Both HCMV IE1-72 and pp65 antigens are highly expressed in craniopharyngioma tissues but not in normal brain tissues. HCMV infection and its antigen expression may be related to the occurrence of craniopharyngiomas. Mechanism needs further study.