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利用大肠杆菌混菌发酵生物合成咖啡醇糖苷。通过在酪氨酸高产大肠杆菌BTAL中表达异源基因酪氨酸解氨酶(tyrosine ammonia-lyase from Rhodotorula glutinis,RgTAL),对羟基肉桂酰辅酶A连接酶(hydroxycinnamate:CoA ligase from Petroselinum crispum,Pc4CL)和肉桂酰辅酶A还原酶(cinnamyl-CoA reductase from Arabidopsis thaliana,AtCCR),与大肠杆菌BL21