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用基因枪法将来自于棉花的DREB转录因子基因和诱导型启动子Rd29A构建的植物表达载体pRdGH转入小麦品种鲁麦22号,获得转基因小麦植株。T0、T1、T2、T3代分子生物学鉴定证实:GH-DREB基因可以稳定遗传。孕穗期到开花期T3代叶片可溶性糖、蒸腾速率、净光合速率测定结果表明:正常条件下,转基因后代与受体鲁麦22的各生理指标无明显差异,而水分胁迫下,转基因后代的净光合速率随着干旱处理时间的延长而逐渐下降,蒸腾速率随着干旱处理时间的延长而逐渐上升,但是,下降与上升幅度比对照低,其中,在胁迫第3天、第6天都与对照差异极显著,叶片的可溶性糖含量与对照相比差异不显著。说明GH-DREB基因在干旱条件下可通过减少蒸腾速率、维持光合作用。
The transgenic wheat plants were obtained by using the gene gun method. The plant expression vector pRdGH constructed from the cotton DREB transcription factor gene and the inducible promoter Rd29A was transformed into the wheat cultivar Luomai22. T0, T1, T2, T3 molecular biology confirmed that: GH-DREB gene can be stably inherited. The results of the soluble sugar, transpiration rate and net photosynthetic rate of T3 generation from booting stage to flowering stage showed that under normal conditions, the physiological indexes of transgenic progeny and recipient Lumai 22 had no significant difference. Under water stress, the net The photosynthetic rate decreased gradually with the prolongation of drought treatment. The transpiration rate increased gradually with the prolongation of drought treatment time. However, the decline and increase rate were lower than the control. Among the three treatments, The difference was significant. The soluble sugar content of leaves was not significantly different from the control. This indicates that GH-DREB gene can maintain photosynthesis by reducing transpiration rate under drought conditions.