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目的 快速分离血液中的细菌。方法 葡聚糖凝聚红细胞后 ,等密度离心 ,上清液用0 .2 2μm微孔滤膜过滤截留细菌、培养。结果 110例血标本采用离心膜滤法和常规血培养法细菌检出率无显著性差异 (χ2 =0 .2 5 ,P >0 .0 5 ) ;该两法检验结果有相关关系 (χ2 =6 1.6 4,P <0 .0 5 ) ,r =0 .82 88;离心膜滤法的细菌检出时间平均为 (2 4.5 5± 9.0 8) h,明显快于常规血培养法 (75 .2 7±12 .11) h(t=16 .5 4,P <0 .0 0 1)。其阳性似然比为 75 .43,阴性似然比为 0 .2 2。结论 离心膜滤法能快速诊断败血症 ,有较高的灵敏度和特异性。
Purpose Rapid separation of bacteria in the blood. Methods After dextran agglutinated erythrocytes, the cells were centrifuged at the same density. The supernatant was filtered through 0.2 2 μm microporous membrane to filter the bacteria and cultured. Results There was no significant difference in the detection rate of bacteria in 110 cases of blood samples by centrifugal membrane filtration and conventional blood culture (χ2 = 0.52, P> 0.05). There was a correlation between the two test results (χ2 = 6 1.6 4, P <0.05), r = 0.82 88; the average time of detection of bacteria by centrifugal membrane filtration was (4.55 ± 9.08) h, which was significantly faster than that of conventional blood culture method (75. 2 7 ± 12 .11) h (t = 16.54, P <0.001). The positive likelihood ratio was 75.43, and the negative likelihood ratio was 0.22. Conclusion Centrifugal membrane filtration can rapidly diagnose sepsis with high sensitivity and specificity.