目的建立HPLC测定痛风舒片中大黄素及大黄酚含量的方法。方法以HPLC法测定痛风舒片中大黄素和大黄酚的含量。Phenomenex C_(18)(250 mm×4.6 mm,5 um)色谱柱为固定相,甲醇-0.05%磷酸(89:11)为流动相,流速1.0 m L/min,检测波长254 nm。结果线性范围大黄素在0.04μg~0.32μg(r=0.99990)、大黄酚在0.57μg~4.52μg(r=0.99994),大黄素平均回收率为97.1%、大黄酚平均回收率为97.4%,两者相对标准偏差分别为大黄素1.25%、大黄酚0.83%(n=5)。结论该方法灵敏度高、专属性强、重现性好,可作为痛风舒片质量控制指标。 OBJECTIVE To establish a HPLC method for the determination of emodin and chrysophanol in Tongfengshu tablets. Methods HPLC method was used to determine the content of emodin and chrysophanol. Phenomenex C_ (18) (250 mm × 4.6 mm, 5 μm) column was used as stationary phase. The mobile phase consisted of methanol-0.05% phosphoric acid (89:11) and the flow rate was 1.0 m L / min. The detection wavelength was 254 nm. Results The average range of emodin was 0.04μg ~ 0.32μg (r = 0.99990), while that of chrysophanol was 0.57μg ~ 4.52μg (r = 0.99994). The average recovery of emodin was 97.1% and the average recovery of chrysophanol was 97.4% The relative standard deviations were 1.25% for emodin and 0.83% for chrysophanol (n = 5). Conclusion The method is sensitive, specific and reproducible, and can be used as a quality control index for gout.