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目的系统分析人类免疫缺陷病毒(HIV)-1DNA疫苗初免-重组痘苗病毒载体(rTV)疫苗和蛋白疫苗加强免疫策略在小鼠中诱导的免疫应答反应,为HIV-1疫苗研发提供备选免疫方案和免疫原性评价手段。方法采用初免-加强策略免疫BALB/c小鼠,ELISA检测特异性结合抗体,流式细胞术检测特异性细胞免疫反应。结果免疫程序中4个时间点血清样本均可持续检测到广泛的HIV-1特异性抗体(IgG、IgM、IgA、IgG1、IgG2a、IgG2b和IgG3);rTV疫苗免疫后10d和末次免疫后6周均可检测到HIV-1特异性的细胞免疫反应,分泌细胞因子干扰素(IFN)-γ、白细胞介素(IL)-2和肿瘤坏死因子(TNF)-α;该免疫策略可诱导广泛的三功能性和双功能性T细胞免疫反应。结论 DNA疫苗初免-rTV疫苗和蛋白疫苗加强免疫策略可以在小鼠中诱导出广泛持久的HIV-1特异性体液免疫和细胞免疫应答。
Objective To systematically analyze the immune response induced in mice by DNA vaccine prime-recombinant vaccinia virus (rTV) vaccine and protein vaccine boosting strategy, and to provide alternative immunization for the development of HIV-1 vaccine Protocol and immunogenicity assessment tools. Methods BALB / c mice were immunized with prime-boost strategy, specific binding antibodies were detected by ELISA, and specific cellular immune responses were detected by flow cytometry. Results A wide range of HIV-1 specific antibodies (IgG, IgM, IgA, IgG1, IgG2a, IgG2b and IgG3) were continuously detected in the serum samples at 4 time points during the immunization program; at 10 days after vaccination and 6 weeks after the last immunization Can detect HIV-1-specific cellular immune responses and secrete cytokine interferon (IFN) -γ, interleukin (IL) -2 and tumor necrosis factor (TNF) -α; this immunization strategy can induce a wide range of Trifunctional and bifunctional T cell immune responses. Conclusions DNA vaccine priming-rTV vaccination and protein vaccination boosting strategies induce extensive and sustained HIV-1 specific humoral and cellular immune responses in mice.