论文部分内容阅读
选用 8 0个随机引物 ,对栽培种陆地棉、野生种雷蒙德氏棉、它们的杂种一代以及从回交后代中选育的 9个种质系进行了RAPD指纹图谱和遗传相似性研究。结果表明 :2 9个随机引物扩增的带具多态性 ,并能将两个不同棉种、F1 和种质系相互间加以区别。在 9个种质系中分别检测到 5～ 9条 (3 .7%～ 5 .3 % )带与野生种相同的特异带 ,大多数带与陆地棉回交亲本一致。野生种与种质系间的相似系数为 0 .388～ 0 .479,陆地棉亲本与种质系间相似系数为 0 .6 36～ 0 .892 ,材料间的带型差异与相似系数是吻合的。因此 ,利用RAPD标记检测远缘杂种后代材料中来自野生种的血缘是可行的。
Eighty random primers were used to study the RAPD fingerprinting and genetic similarity of cultivated species of G. hirsutum L., G. mongolica, their hybrids and 9 germplasm lines selected from backcross progenies. The results showed that there were polymorphic bands amplified by 29 random primers and could distinguish two different cotton varieties, F1 and germplasm lines from each other. In 9 germplasm lines, 5 ~ 9 (3. 7% ~ 5. 3%) bands were detected with the same specific bands as the wild species, most of the bands were consistent with the upland cotton backcross parents. The similarity coefficient between wild species and germplasm ranged from 0.388 to 0.489. The similarity coefficient between G. napus parents and germplasm ranged from 0.636 to 0.892. The differences between the two species were in accordance with the similarities of. Therefore, the use of RAPD markers to detect distant hybrid progeny from the blood of wild species is feasible.