论文部分内容阅读
目的探讨博尔纳病病毒(BDV)在宁夏及其周边地区的流行状况。方法采用巢式逆转录酶聚合酶链反应结合荧光定量PCR检测了52例病毒性脑炎(VE)患者和32名健康人外周血液(PBMC)和脑脊液有核细胞(CSFMC)、53例抑郁症(DD)患者和360只绵羊PBMC中BDV p24基因片段,对阳性产物进行基因序列测定、同源性和氨基酸顺序分析;并绘制系统发生树,分析BDV感染的分子流行病学特点。结果BDV p24基因片段阳性率在VE患者CSFMC为11．54％、DD患者PBMC 11．32％明显高于健康人0％(P<0．05);绵羊的PBMC为7．78％与健康人对比差异无统计学意义(P>0．05);系统发生树图形表明,绵羊PBMC目的基因片段与人类VE和DD核苷酸序列亲缘关系最近,来源于一个分支,并与德国H1766病毒株核苷酸序列亲缘关系最近,其变异性较小。结论宁夏及其周边地区部分VE、DD可能与BDV感染有关,健康绵羊存在BDV的自然感染,其流行可能具有一定的地域局限性;人类VE、DD的BDV感染可能存在潜在动物源性,有待于进一步研究。
Objective To investigate the prevalence of Borna disease virus (BDV) in Ningxia and its surrounding areas. Methods 52 cases of viral encephalitis (VE) and 32 healthy people (PBMC), CSF CSF, 53 cases of depression were detected by nested reverse transcriptase polymerase chain reaction and real-time PCR. (DD) patients and 360 sheep PBMC p24 gene fragment of BDV gene products were sequenced, homology and amino acid sequence analysis; and phylogenetic tree analysis of BDV infection molecular epidemiological characteristics. Results The positive rate of BDV p24 gene fragment in CSF was 11.54% in VE patients and 11.32% in DD patients was significantly higher than that in healthy subjects (P <0.05). The percentage of PBMC in sheep was 7.78% (P> 0.05). The phylogenetic tree pattern indicated that the PBMC gene fragment of sheep had the closest genetic relationship with human VE and DD nucleotide sequences, originated from a branch and was associated with the German H1766 strain The nucleotide sequence has the closest genetic relationship with less variability. Conclusion Some VE and DD in Ningxia and its surrounding areas may be related to BDV infection. Natural infection with BDV in healthy sheep may have some epidemiological limitations. BDV infection in human VE and DD may have potential animal origin, further research.