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A novel method for analysis of three active components curcumin,demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L.was developed by HPLC coupled with electrochemical detection.Three curcuminoids were well separated on a C_(18) column and detected with high sensitivity.A mobile phase containing acetonilrile and 10 mM Na_2HPO_4-H_3PO_4(pH 5.0)(50:50.v/v) was used.Good linearity was obtained in the range of 0.208-41.6,0.197-39.4,and 0.227-114 μM for curcumin.demethoxycurcumin and bisdemethoxycurcumin respectively.The limit of detection reached up to 10~(-8) M.which was lower than that by UV detection.The relative standard deviations(RSDs) ranged from 1.06% to 1.88% for intra-day precision and from 4.30% to 5.79% for inter-day precision,respectively.The proposed method has been applied in real herb sample and recoveries ranging from 86.3% to 111% were obtained.
A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L.was developed by HPLC coupled with electrochemical detection. Curcuminoids were well separated on a C 18 column and detected with high sensitivity. A mobile phase containing acetonilrile and 10 mM Na 2 HPO 4 -H 3 PO 4 (pH 5.0) (50: 50. v / v) was used. Good linearity was obtained in the range of 0.208-41.6, 0.97-39.4, and 0.227-114 μM for curcumin.demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10 -8 M.which was lower than that by UV detection. The relative standard deviations (RSDs) ranged from 1.06% to 1.88% for intra-day precision and from 4.30% to 5.79% for inter-day precision, respectively. This proposed method has been applied in real herb sample and recoveries ranging from 86.3% to 111% were obtained.