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The reactions of four different N-(O,O′-diisopropyl) phosphoamino acids (DIPP-aa),such as N-phosphoryl-L-α-alanine (DIPP- L-α-Ala),N-phosphoryl-D-α-alanine (DIPP-D-α-Ala),N-phosphoryl-β-alanine (DIPP-β-Ala) and N-phosphoryl-γ-amino butyric acid (DIPP-γ-Aba),and four nucleosides,adenosine (A),guanosine (G),cytidinc (C) and uridine (U),were studied by electrospray ionization tandem mass spectrometry (ESI-MS/MS) and HPLC/ESI-MS.DIPP-L-α-Ala and DIPP-D-α-Ala produced the same phosphorylated nucleosidcs,dinucleotidcs and phosphoroligopeptide.However,DIPP-β-Ala and DIPP-γ-Aba gave no relevant products.
The reactions of four different N- (O, O’-diisopropyl) phosphoamino acids (DIPP-aa), such as N-phosphoryl- L- alpha-alanine (DIPP- α-alanine (DIPP-D-α-Ala), N-phosphoryl-β-alanine (DIPP-β-Ala) and N-phosphoryl- γ-amino butyric acid (DIPP-γ- Aba), and four nucleosides, adenosine (A), guanosine (G), cytidinc (C) and uridine (U), studied by electrospray ionization tandem mass spectrometry (ESI-MS / MS) and HPLC / ESI- -D-α-Ala produced the same phosphorylated nucleosidcs, dinucleotidcs and phosphoroligopeptide. Still, DIPP-β-Ala and DIPP-γ-Aba gave no relevant products.