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目的测量正常人的房水闪辉和细胞数量,观察年龄和性别对血-房水屏障的影响。设计前瞻性病例系列研究。研究对象221例正常人(442眼)。方法采用FC-2000型激光蛋白细胞检测仪(LFCM)非接触、定量地检测了221例正常人(442眼)的房水闪辉和细胞。主要指标房水闪辉和细胞。结果正常人的房水闪辉平均值为(4.7±2.9)pc/ms,其中<10岁为(3.1±1.O)pc/ ms,40-49岁组为(3.8±1.9)pc/ms,≥80岁者达到(11.0±4.8)pc/ms。与40～49岁组相比,50岁以上各年龄组的房水闪辉值均显著增高(P均<0.05),男性与女性之间无显著差异(P=0.686),左右眼之间无显著差异(P=0.512)。正常人房水细胞平均值为(0.4±0.7)个,与40～49岁年龄组相比,80岁以上者房水细胞值显著增高(P=0.014),男性与女性之间无显著差异(P=0.413),左右眼之间无显著差异(P=0.886)。结论LFCM可定量检测房水闪辉值并了解蛋白浓度和细胞数量,此项检查对定量检测血-房水屏障的改变和前房炎症具有重要意义。
Objective To measure the amount of atrial fibrillation and cell number in normal subjects and observe the effect of age and gender on blood-aqueous barrier. Design prospective case series. 221 healthy subjects (442 eyes). Methods Non-contact and quantitative detection of aqueous humor and cells in 221 normal subjects (442 eyes) by FC-2000 laser protein cell detector (LFCM) was performed. The main indicator of aqueous shine and cells. Results The mean value of atrial flashover in normal subjects was (4.7 ± 2.9) pc / ms, which was (3.1 ± 1.0) pc / ms for <10 years and (3.8 ± 1.9) pc / ms for 40-49 years (11.0 ± 4.8) pc / ms for ≥80 years old. Compared with those aged 40-49 years, the atrophy of aqueous humor in each age group over 50 years old was significantly higher (P <0.05), while there was no significant difference between males and females (P = 0.686). There was no significant difference between the left and right eyes Significant difference (P = 0.512). The average number of aqueous humor cells in normal people was (0.4 ± 0.7). Compared with the age group of 40-49, the values of aqueous humor cells in patients over 80 years old were significantly increased (P = 0.014), while there was no significant difference between men and women P = 0.413), no significant difference between left and right eyes (P = 0.886). Conclusions LFCM can quantitatively detect the brightness of aqueous humor and understand the protein concentration and cell number. This test is of great significance for the quantitative detection of blood-aqueous humor barrier and inflammation of the anterior chamber.