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目的了解慢性肾功能衰竭患者外周血单核细胞向树突状细胞(DC)分化的能力。方法采用流式细胞仪检测细胞表面标记表达,混合淋巴细胞反应评价细胞的抗原递呈能力。结果慢性肾功能衰竭患者外周血单核细胞能向DC分化,分化而成的DC表达CD11c,CD40,CD80,CD83的阳性率显著低于正常人(P<0.05),刺激淋巴细胞增殖的能力显著弱于正常人(P<0.01)。结论慢性肾功能衰竭患者外周血单核细胞向DC分化的能力减弱,分化而成的DC具有较弱的抗原递呈能力。
Objective To investigate the ability of peripheral blood mononuclear cells to differentiate into dendritic cells (DCs) in patients with chronic renal failure. Methods Flow cytometry was used to detect the expression of cell surface markers and mixed lymphocyte reaction to evaluate the antigen presenting ability of cells. Results The peripheral blood mononuclear cells of chronic renal failure patients could differentiate into DCs. The positive rate of CD11c, CD40, CD80 and CD83 expression in differentiated DCs was significantly lower than that in normal persons (P <0.05), and the ability of stimulating lymphocyte proliferation was significant Weaker than normal people (P <0.01). Conclusion The ability of peripheral blood mononuclear cells to differentiate into DC is weakened in patients with chronic renal failure. Differentiated DCs have weaker antigen presenting ability.