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目的:评价前S_1蛋白在乙型病毒性肝炎早期诊断、疗效及预后等方面的作用。方法:用乙型肝炎病毒(HBV)前S_1合成肽及其免疫血清建立前S_1抗原和抗体ELISA检测方法,分别检测219份急性乙型肝炎(AHB)患者(44例)系列血清、535份慢性肝炎患者及HBsAg携带者系列血清和63份HBsAg阳性患者血清。HBV DNA检测采用核酸斑点杂交法和PCR法,其余指标检测采用ELISA法。结果:前S_1抗体在血液中存在时间短暂,前S_1抗原阴转越早、前S_1抗体阳转越早,AHB患者的病程越短、预后越好。结论:前S.抗体有可能成为AHB的早期诊断指标;前S_1抗原为反映HBV复制和抗HBV疗效的一个敏感指标。
Objective: To evaluate the role of pre S_1 protein in the early diagnosis, curative effect and prognosis of hepatitis B virus. Methods: The pre-S_1 antigen and antibody were detected by ELISA of pre-S_1 synthetic peptide of hepatitis B virus (HBV) and its immune serum. Serum samples from 219 patients with acute hepatitis B (AHB), serum samples of 535 Hepatitis and HBsAg carriers sera and 63 HBsAg positive sera. HBV DNA detection using nucleic acid dot blot hybridization and PCR, the remaining indicators detected by ELISA. Results: The former S_1 antibody existed in the blood for a short period of time, the earlier the former S_1 antigen turned negatively, the earlier the positive S_1 antibody became positive and the shorter the duration of AHB was, the better the prognosis was. Conclusion: The former S. pneumoniae antibody may be the early diagnosis index of AHB. The former S_1 antigen is a sensitive index to reflect the effect of HBV replication and anti-HBV.