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目的:采用超高效液相(UPLC)及亲水作用色谱法(HILIC,hydrophilic interaction chromatography)测定半夏中胡芦巴碱的含量,建立半夏药材质量评价方法。方法:半夏细粉经50%的甲醇超声提取后,采用ACQUITY UPLC BEH HILIC色谱柱(2.1 mm×100 mm,1.7μm)进行胡芦巴碱含量的测定,流动相为乙腈-10 mmol.L-1醋酸铵(80∶20),流速0.4 mL.min-1,检测波长265 nm,进样量10μL。结果:盐酸胡芦巴碱在1.125～72 nmol.mL-1线性关系良好(r=0.999 6,n=7),平均回收率为99.7%(RSD 1.4%)。结论:该方法专属性强、重复性好、稳定、可控,可作为半夏药材质量控制的方法。
OBJECTIVE: To determine content of trigonelline in Pinellia ternata by UPLC and hydrophilic interaction chromatography (HICC), and to establish a method for evaluating its quality. Methods: The content of trigonella was determined by ACQUITY UPLC BEH HILIC column (2.1 mm × 100 mm, 1.7 μm) after 50% methanol was extracted by 50% methanol. The mobile phase was acetonitrile-10 mmol·L -1 ammonium acetate (80:20), the flow rate of 0.4 mL.min-1, the detection wavelength of 265 nm, the injection volume of 10 μL. Results: Trigone hydrochloride had a good linearity (r = 0.999 6, n = 7) at a linear range of 1.125 ~ 72 nmol.mL-1 with an average recovery of 99.7% (RSD 1.4%). Conclusion: The method is highly specific, reproducible, stable and controllable, and can be used as a method for quality control of Pinellia ternata.