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建立反相液相色谱法测定大鼠血浆中胡黄连苷Ⅰ(HD-Ⅰ)的含量,并对胡黄连苷-Ⅰ在大鼠体内动力学特征进行分析研究。以芍药苷为内标物,血浆样品经Vc抗氧化,乙腈沉蛋白后,采用AgilentXDBC18色谱柱(250mm×4.6mm,5μm)分离,流动相:乙腈-水,梯度洗脱程序[(0-1)min]20:80,[(1-2)min]24:76,[(2-6)min]24:76,[(6-8)min]20:80,([(8-14)]min20:80;检测波长:277nm。结果:HD-Ⅰ线性范围(0.1-150)μg/mL(r=0.9994),最低检测浓度25ng,回收率分别为(94.70±3.27)％、(95.09±0.78)％、(95.91±0.83)％,日内、日问精密度准确度均小于10％。大鼠单次静脉注射HD-Ⅰ7mg/kg、14mg/kg、40mg/kg后,血药浓度-时间曲线均呈二室开放模型,半衰期T1/2β分别为(27.07±6.56)min、(29.78±6.24)min、(31.41±4.75)min,清除速率CL(s)值分别为(0.050±0.017),(0.049±0.012),(0.046±0.025)mg·kg-1·min-1(μg·mL-1),平均滞留时间MRT平均为(15.86±0.29)min,曲线下面积AUC和AUC(so)与剂量呈正相关。证实方法准确、灵敏度高,可用于HD-Ⅰ体内过程的研究。HD-Ⅰ在大鼠体内符台线性动力学二室开放模型,代谢快,分布广,清除速率快。
A reversed-phase high performance liquid chromatography method was developed to determine the content of picroside I (HD-I) in rat plasma. The dynamic characteristics of picroside-I in rats were analyzed. With peoniside as internal standard, plasma samples were anti-oxidized by Vc, and acetonitrile was separated using Agilent XDBC18 column (250mm×4.6mm, 5μm). Mobile phase: acetonitrile-water, gradient elution procedure [(0-1 ]min]20:80,[(1-2)min]24:76,[(2-6)min]24:76,[(6-8)min]20:80,([(8-14) ]min 20:80; detection wavelength: 277 nm. Results: HD-I linear range (0.1-150) μg/mL (r = 0.9994), the lowest detection concentration 25 ng, recovery was (94.70 ± 3.27)%, (95.09 ± 0.78)% and (95.91±0.83)%, precision of intra-day and daily-interval precision was less than 10%. After a single intravenous injection of HD-I7mg/kg, 14mg/kg, and 40mg/kg in rats, plasma concentration-time The curves are presented as a two-compartment open model, with half-life T1/2β being (27.07±6.56) min, (29.78±6.24) min, (31.41±4.75) min, and clearance CL(s) values being (0.050±0.017), respectively. (0.049±0.012), (0.046±0.025) mg·kg-1·min-1 (μg·mL-1), mean residence time MRT averaged (15.86±0.29) min, area under curve AUC and AUC(so) It is positively correlated with the dose.The method is accurate and has high sensitivity, and can be used for the study of HD-I in vivo process.HD-I is a two-compartment open model of linear dynamics in rat body, with fast metabolism, Wide, fast clearance rate.