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2009年春秋2次试验旨在比较基质育苗裸苗移栽、水浮育苗带基质移栽和无土育苗无钵移栽3种方法缓苗期棉苗生理生化指标的差异。2次试验育苗均在温室进行,春季试验幼苗移栽大田采用完全随机区组设计,秋季采用微区设计,均为3次重复。结果表明:3种育苗方法2次试验均因移栽时产生的逆境胁迫而使叶绿素含量、MDA含量在缓苗期内呈现先急剧升高而后迅速下降再趋于平稳的变化规律。缓苗期内SOD、POD、CAT三种酶活性均为先上升后下降再稳定于某个水平,且三者呈现一定的相关性。基质苗从缓苗后期开始,新根生长迅速且在取样结束前总长高于无钵苗,低于水浮苗,但三者无显著性差异。基质苗的干重和叶面积介于水浮苗与无钵苗之间。
The two trials in spring and autumn in 2009 were aimed to compare the differences of physiological and biochemical indexes of three kinds of cotton seedling during recuperation on transplanting of nursery seedling with substrate nursery stock, substrate transplanting with floating nursery stock and soilless transplant with soilless nursery. Two trials of nursery are carried out in the greenhouse, the spring test seedlings transplanted field using a completely randomized block design, the autumn design by micro-area, were 3 replications. The results showed that the chlorophyll content and MDA content of the three seedling raising methods in the two experiments both increased sharply and then decreased sharply and then stabilized after the transplanting. The activities of SOD, POD and CAT during the rejuvenation period were first increased, then decreased and then stabilized at a certain level, and the three showed a certain correlation. The seedlings grew rapidly from the late stage of Huanmiao and the new roots grew rapidly. The total length of the seedlings before the end of the sampling was higher than that without the seedlings, but lower than that of the seedling. However, the three showed no significant difference. The dry weight and leaf area of the substrate seedlings ranged between the floating seedlings and the seedling-free seedlings.