论文部分内容阅读
本文采取补体依赖微量细胞毒方法筛选HLA抗血清。一般以产妇血为筛选对象,20～100个随机个体淋巴细胞为靶细胞进行筛选。由于通常产妇血中含有的HLA抗体总是针对其丈夫白细胞HLA抗原,这是因为夫妇间HLA差异通过胎母免疫而产生的抗体。故本文可以丈夫外周血T、B淋巴细胞取代随机个体细胞作靶抗原来检测。共筛选了312例妊娠妇女,检出阳性血清44例(14.10％),其中强阳性20例(6.41％),较—般随机细胞为靶抗原阳性检出率为高。由于检测时作了T、B细胞分离,故结果显示了含HLA-A、B、C抗血清34份,可能含DR抗血清19份。在此同时对其中30例产妇同时用36随机个体细胞为靶抗原,4例产妇用其胎儿脐带血T、B淋巴细胞为靶抗原进行检测以作对照,三组结果显示非常相关,无显著差异。这表明了以丈夫血细胞为靶抗原筛选HLA抗血清方法简单,不需要大量随机个体细胞,灵敏度高,因此宜在常规筛选HLA抗血清的实验室,特别是在产科医院中推广施行。
In this paper, complement-dependent micro-cytotoxicity screening of HLA antiserum. Generally maternal blood for the screening of objects, 20 to 100 random individual lymphocytes to target cells for screening. Since the maternal blood usually contains HLA antibodies that are always directed against their husband’s leukocyte HLA antigens, this is due to the difference in HLA antibodies produced by the maternal immunization between couples. Therefore, this article can her husband’s peripheral blood T, B lymphocytes instead of random individual cells as the target antigen to detect. A total of 312 pregnant women were screened, and 44 cases (14.10%) were detected positive serum, of which 20 cases were strongly positive (6.41%). The detection rate was higher than that of random cells. As a result of testing made of T, B cell separation, the results showed that with HLA-A, B, C anti-serum 34, may contain 19 anti-serum DR. At the same time, 30 cases of maternal at the same time with 36 random individual cells as the target antigen, 4 cases of maternal fetal cord blood T, B lymphocytes as the target antigen for testing as controls, the three groups showed very relevant results, no significant difference . This shows that the method of screening HLA antisera using husband blood cells as target antigen is simple, does not require a large number of random individual cells and has high sensitivity, and therefore should be popularized in laboratories that routinely screen for HLA antisera, especially in obstetric hospitals.