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目的:比较研究顺序特异引物聚合酶链反应(PCR-SSP)方法与标准血清学方法对人类白细胞抗原-DR(HIA-DR)分型的精确性和临床应用价值.方法:选择肾移植受者101例,供者61例,采用PCR-SSP和血清学方法同步行HLA-DR分裂,比较其检测时间、敏感性、特异性和临床实用性.结果:162份样本,PCR-SSP分型均获成功,检出DR等位基因总数308个,分型时间5小时,特异性和重复性100%.血清学分型耗时20小时,8个位点不肯定,29个分型错误,35个空白位点中20个存在第二个位点,总误差率30.2%.结论:PCR-SSP方法用于HLA-DR分型较血清学方法快速、精确,试剂与临床样本易得,适合于临床应用.
OBJECTIVE: To compare the accuracy and clinical value of sequence-specific primer-polymerase chain reaction (PCR-SSP) and standard serological methods in the classification of human leukocyte antigen-DR (HIA-DR) 101 cases and 61 donors were selected.The HLA-DR scores were analyzed simultaneously by PCR-SSP and serological methods.Results: The results of PCR-SSP, PCR-SSP and PCR- The number of DR alleles was 308, the typing time was 5 hours, specificity and reproducibility was 100%, the serological typing time was 20 hours, 8 loci were not certain, 29 typing errors and 35 There were 20 second sites in the blank sites, with a total error rate of 30.2% .Conclusion: The PCR-SSP method for HLA-DR typing is faster and more accurate than serological methods and is readily available for clinical reagents and reagents application.