水稻离体茎穗室内杂交技术最先为广东省农科院创造。他们在田间取回茎穗(不带根)在室内杂交后,将母本茎穗植于人工配制的培养液中,于有散射光的房间内让其发育结实直至成熟。后来贵州农学院吴平理等对此方法作了改进,一改为母本离体茎穗带根泥土栽培。我们在此基础上改进了父本 In vitro hybridization of stems and leaves of rice was first created by Guangdong Provincial Academy of Agricultural Sciences. After retrieving the stem spikes (without roots) in the field after they have been crossed indoors, they sprout their maternal spikes into artificially prepared culture broths and allow them to develop sturdily until matured in a room with scattered light. Later, Wu Ping Li, Guizhou Agricultural College and other methods to improve this, changed to the female parent root stalk sprout cultivation. On this basis, we have improved the patriarch