论文部分内容阅读
目的观察矢车菊素-3-葡萄糖苷(cyanidin-3-glucoside,C3G)对肥胖大鼠血清炎症因子(TNF-α、IL-6和MCP-1)及胰岛素敏感性的影响。方法 3周龄雄性SD大鼠30只,随机分为对照组(n=8)和高脂饮食组(n=22),分别予以普通饲料及高脂饲料喂养5周。肥胖造模成功的17只大鼠再随机分为肥胖组(n=8)和C3G组(n=9)。C3G组予C3G100mg/(kg·d)灌胃,其余组予等量生理盐水灌胃。实验结束时准确称量大鼠体重及内脏脂肪质量,全自动生化分析仪测定空腹血糖(fasting glucose,FPG),放免法测血清胰岛素(fasting insulin,FINS),ELISA测血清脂联素及TNF-α、IL-6、MCP-1水平,并计算内脏脂肪比和胰岛素敏感指数(insulin sensitivity index,IAI)。结果肥胖组和C3G组大鼠体重、内脏脂肪比和血清TNF-α、IL-6、MCP-1水平明显高于对照组(P均<0.05),而C3G组上述指标明显低于肥胖组(P均<0.05)。肥胖组和C3G组血清脂联素水平及IAI明显低于对照组(P均<0.05),而C3G组上述指标明显高于肥胖组上述指标。结论 C3G能明显增加肥胖大鼠的胰岛素敏感性,其可能与增加血清脂联素水平及降低血清炎症因子(TNF-α、IL-6、MCP-1)水平有关。
Objective To observe the effects of cyanidin-3-glucoside (C3G) on serum inflammatory cytokines (TNF-α, IL-6 and MCP-1) and insulin sensitivity in obese rats. Methods Thirty male SD rats of 3 weeks old were randomly divided into control group (n = 8) and high fat diet group (n = 22), fed with normal diet and high fat diet for 5 weeks respectively. Seventeen rats with obesity were randomly divided into obesity group (n = 8) and C3G group (n = 9). C3G group given C3G100mg / (kg · d) gavage, and the remaining group were given the same amount of saline gavage. The body weight and visceral fat mass were weighed accurately at the end of the experiment. Fasting glucose (FPG), fasting insulin (FINS), serum adiponectin and TNF- α, IL-6 and MCP-1 levels were measured and visceral fat ratio and insulin sensitivity index (IAI) were calculated. Results The body weight, visceral fat ratio and serum levels of TNF-α, IL-6 and MCP-1 in obese and C3G groups were significantly higher than those in control group (all P <0.05), while those in C3G group were significantly lower than those in obese group P <0.05). Serum levels of adiponectin and IAI in obesity group and C3G group were significantly lower than those in control group (all P <0.05), while those in C3G group were significantly higher than those in obesity group. Conclusion C3G can significantly increase insulin sensitivity in obese rats, which may be related to increasing serum adiponectin level and decreasing serum levels of inflammatory cytokines (TNF-α, IL-6 and MCP-1).