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目的:研究HLA-Ⅰ类基因单倍型与丙型肝炎病毒感染的相关性。方法:选取丙型肝炎病毒抗体和核酸检测均为阳性的献血者426名和同时期献血的健康献血者709名作为实验对象,PCR-SBT法检测HLA-A、B位点等位基因型,利用Helixtree软件计算HLA-A-B单倍型,保留频率>1%的单倍型并对其在HCV感染组与健康对照组中分布进行对比分析。结果:共有22条频率>1%的HLA-Ⅰ类基因单倍型,5条单倍型在HCV感染组的频率显著高于健康对照组,3条单倍型在对照组的分布显著高于感染组,其中HLA-A*11:01-B*15:02在对照组和感染组的频率分别为8.04%和4.46%(P=0.019),OR=0.643,95%CI 0.430~0.962。其余几条低频率单倍型在感染组或对照组频数为0,无法计算OR值。结论:HLA-Ⅰ类基因单倍型与HCV感染存在关联,其中HLA-A*11:01-B*15:02与丙型肝炎病毒感染风险呈负相关。
Objective: To study the association between haplotypes of HLA-Ⅰ gene and hepatitis C virus infection. Methods: A total of 426 blood donors with positive hepatitis C virus and nucleic acid and 709 healthy blood donors were enrolled in this study. The alleles of HLA-A and B loci were detected by PCR-SBT. The HLA-AB haplotype was calculated by Helixtree software and haplotypes> 1% were retained and their distribution in HCV-infected and healthy controls was compared. Results: A total of 22 HLA-Ⅰ haplotypes were found with frequency> 1%. The frequency of 5 haplotypes in HCV-infected group was significantly higher than that in healthy controls. The distribution of 3 haplotypes in control group was significantly higher than that in control group In the infection group, the frequencies of HLA-A * 11: 01-B * 15: 02 in control group and infected group were 8.04% and 4.46% respectively (P = 0.019), OR = 0.643, 95% CI 0.430-0.962. The remaining few low-frequency haplotypes in infected or control group frequency of 0, can not calculate the OR value. Conclusion: HLA haplotypes are associated with HCV infection. HLA-A * 11: 01-B * 15: 02 is negatively correlated with the risk of hepatitis C virus infection.