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为探究镉污染对火龙果的毒性效应,本实验用无菌水冲洗3遍,将种子分别铺于含添加溶液至终浓度为氯化镉0、2μmol/L、5μmol/L、10μmol/L、20μmol/L、40μmol/L、80μmol/L、120μmol/L、200μmol/L、300μmol/L的无菌培养皿内,培养皿内铺有双层滤纸并且培养皿置于人工气候箱培养,每隔24 h更换一次培养液并记录不同浓度每天的种子萌发数,待培养到第7天记录火龙果幼苗根长和株高,进行发芽率、发芽指数、活力指数、POD、SOD的测定。结果表明火龙果种子在不同浓度的Cd溶液下活力指数、发芽率都受到影响,Cd浓度越来越高时,火龙果发芽率越低,生长受到抑制;其中,火龙果子叶的抗氧化性酶在种子生长过程中受镉离子影响下发挥重要作用,Cd浓度越高,POD、SOD、CAT活性降低,从结果可以看出可植物在受到重金属污染的时候,酶含量的变化可能是植物耐重金属的机制之一。
In order to explore the toxic effect of cadmium pollution on dragon fruit, the experiment was rinsed three times with sterile water and the seeds were plated in the solution containing cadmium chloride 0,2μmol / L, 5μmol / L, 10μmol / L, 20μmol / L, 40μmol / L, 80μmol / L, 120μmol / L, 200μmol / L, 300μmol / L sterile petri dishes were plated with double layers of filter paper and the petri dishes were placed in an artificial climate chamber. The culture medium was changed every 24 h, the number of germination per day was recorded, and the root length and plant height of pitaya seedlings were recorded on the 7th day. The germination rate, germination index, vitality index, POD and SOD were measured. The results showed that the vigor index and the germination rate of pitaya seeds were affected under different concentrations of Cd solution. When the Cd concentration was higher and higher, the germination rate of pitaya was lower and the growth was inhibited. Among them, the antioxidant enzyme In the process of seed growth under the influence of cadmium ions play an important role, the higher the Cd concentration, POD, SOD, CAT activity decreased, the plant can be seen from the heavy metal pollution, the changes in enzyme content may be plant resistance to heavy metals One of the mechanisms.