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为阐明人体组织处于生理及病理状态时合成及分泌的各种甲种胎儿蛋白(AFP)分子中糖基组成上的差异,本文采用刀豆球蛋白A(ConA)及扁豆凝集素(LCA)亲和双向放射免疫电泳(Aff-RIEP)分析人体羊水、孕妇、急性肝炎及原发性肝癌患者血清中各种不同来源的AFP。实验表明孕妇及肝炎血清中AFP在ConA及LCA电泳中呈现相似的电泳图谱,但与肝癌AFP有显著差异。在ConA电泳中,肝癌血清显示二种分子变异体,其中以ConA结合型为主,兼有小部分非结合型AFP,而肝炎及孕妇AFP仅为一种ConA结合型分子。然而,在LCA电泳中,肝炎及孕妇血清AFP却以LCA非结合型为主,兼有小部分结合型,但肝癌AFP却与之相反,以结合型为主,非结合型为次。上述结果提示癌性与非癌性AFP分子之间存在糖基组成及结构上的差异。
In order to elucidate the differences of glycosylation among various AFP molecules synthesized and secreted by human tissues in physiological and pathological conditions, ConA and LCA And Affirmatory Radioimmunoassay (Aff-RIEP) were used to analyze various sources of AFP in sera from human amniotic fluid, pregnant women, acute hepatitis and primary liver cancer patients. Experiments show that pregnant women and hepatitis AFP in ConA and LCA electrophoresis showed a similar electrophoresis, but with a significant difference between liver cancer AFP. In ConA electrophoresis, liver cancer sera showed two molecular variants, of which ConA-based, with a small part of non-binding AFP, hepatitis and pregnant women AFP only ConA-binding molecules. However, in the LCA electrophoresis, hepatitis A and pregnant women serum AFP mainly LCA non-binding type, both with a small amount of binding type, but the liver cancer AFP is the opposite, the binding type, non-binding type times. The above results suggest that the glycosylation and structural differences exist between cancerous and non-cancerous AFP molecules.