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目的 分析贵州省狂犬病病毒的糖蛋白基因(G基因)序列特征,为狂犬病的有效预防和控制提供科学依据.方法 以RT- PCR扩增贵州省近年来不同地区的人和犬狂犬病病毒阳性脑组织标本狂犬病病毒G基因序列,对扩增产物测序后采用生物信息学软件进行序列分析.结果 贵州省狂犬病毒阳性标本经RT-PCR扩增、测序与拼接后得到8份狂犬病病毒阳性样本的G基因序列.同源性分析显示.8份狂犬病病毒阳性样本的G基因序列在核苷酸及氩基酸水平上彼此的同源性分别为87.4％～99.9％和83.3.％～100％,与狂犬病毒基因1型病毒株G基因的核苷酸和氨基酸序列同源性最高(分别为86.5％～87.O％和83.3％～100％).进化树分析显示,贵州省狂犬病病毒G基因的亲缘进化关系较近,且与狂犬病毒基因1～7型中的基因1型代表毒株的亲缘进化关系最近；除GZ01和GZ09外,其余狂犬病毒G基因与国内湖北、湖南、安徽、广西、江苏和上海毒株相距较近.除GZ09与国外的马来西亚和泰国代表毒株亲缘进化关系最近外,其与毒株与印度尼西亚毒株亲缘进化关系最近.结论 本研究从狂犬病病毒G基因水平证明贵州省狂犬病毒株属于基因l型,揭示了我省贵州省狂犬病毒与国内外已报道毒株的亲缘进化关系,该研究结果将为贵州省狂犬病的有效预防和控制提供科学依据.“,”To study the characteristic of glycoprotein gene sequence of rabies virus in Guizhou Province in recent years and provide scientific basis for effective control and prevention of rabies,RT-PCR assay were used to detect human and dog brain tissues derived from different prefectures of Guizhou.The amplification products were sequenced and analyzed with bioinformatics software.The results showed that the full-length G gene sequences of 8 positive samples were obtained by RT-PCR amplification,sequencing and splicing.The homology of eight G gene sequences from Guizhou Province were between 87.4％ -99.9％ and 83.3.％- 100％ on nucleotide and deduced amino acid level,respectively,and the highest homology were found with the genotype 1 strains ( 86.5 ％- 87.0％ for nucleotide and 83.3 ％- 100 ％ for amino acid) among genotype 1- 7 representative strains.Besides,phylogenetic analysis based on the G gene indicated that the relationship of 8 strains derived from Guizhou were closest to genotype 1 Lyssavirus,and the strains of Guizhou were very close to the strains come from Hubei,Hunan,Anhui,Guangxi,Jiangsu provinces and Shanghai,except for GZ01 and GZ09.Moreover,GZ09 were evolutionarily closed to the strains come from Malaysia and Thailand,while the remaining sequences were closed to the strain of Indonesia.This study confirmed on the G gene level that rabies virus epidemic strains circulated in Guizhou Province in recent years belonged to rabies virus genotype 1 and the evolutionary relationship with reported strains come from different provinces of China and different countries were revealed in this study.It will provide scientific basis for effective control and prevention of human and animal rabies in Guizhou Province.